Tian Xue, Ph.D.
Professor, School of Life Sciences
University of Science & Technology of China
443 Huangshan Street, Hefei City, Anhui 230027, P.R.China
Sensing light signals provides us with visual perception (image vision) but also regulates many important physiological functions such as circadian-rhythm photoentrainment, pupillary light reflex, sleep, locomotion and secretion of melatonin – functions referred to collectively as non-image vision. It had been demonstrated that a small subset (~1%) of retinal ganglion cells (ipRGCs) can sense light by themselves for non-image vision, by expression of melanopsin, a recently discovered visual pigment. Our lab has broad interests in both image and non-image visions. My previous work includes the elucidation of the key phototransduction components in the ipRGCs.
based on this framework, we would like to delve into the next level of details regarding melanopsin phototransduction process, including the second messengers, any modulation or negative-feedback control, and the underlying molecular mechanisms. In particular, as a detector of ambient light level, ipRGCs have an important feature for having light responses that last much longer than the rods and cones. We would like to understand the underlying mechanisms. In addition, by using multiphoton in vivo imaging, we would like to decipher the neuronal information processing of non-image vision functions in the brain.
A separate goal of the lab is trying to regenerate rod/cone photoreceptors to restore image-vision function in retinal degeneration caused by diverse diseases. We have examined the light response for the newborn rod photoreceptors differentiated from transplanted retinal precursor cells (collaboration with Robin Ali’s group in UK). We will combine our strength in cellular light-sensor physiology with stem-cell techniques, to improve photoreceptors regeneration and their functional integration.
REPRESENTATIVE PUBLICATIONS (12 out of 21)
1) Pearson RA, Barber AC, Rizzi M, Hippert C, Xue T, West EL, Duran Y, Smith AJ, Chuang JZ, Azam SA, Luhmann UFO, Benucci A, Sung CH, Carandini M, Yau KW, Sowden JC, Ali RR. (2012). estoration of vision after transplantation of photoreceptors. Nature. 485(7396):99-103.
2) Xue T¶, Do MT, Riccio A, Jiang Z, Hsieh J, Wang HC, Merbs SL, Welsbie DS, Yoshioka T, Weissgerber P, Stolz S, Flockerzi V, Freichel M, Simon MI, Clapham DE, Yau KW¶. (2011). Melanopsin Signaling in Mammalian Iris and Retina. Nature. 479(7371):67-73. (Research Article) (¶ co-corresponding authors); Faculty of 1000: f1000.com/13360986.
3) Do MT, Kang SH, Xue T, Zhong H, Liao HW, Bergles DE, Yau KW. (2009) Photon capture and signalling by melanopsin retinal ganglion cells. Nature. 457(7227):281-7. (Research Article); Faculty of 1000: f1000.com/1144855.
4) Luo DG, Xue T, Yau KW. (2008) How vision begins: An odyssey. Proc Natl Acad Sci U S A. 105(29): 9855-62 (review)
5) Fu YB*, Kefalov V*, Luo DG*, Xue T*, Yau KW. (2008) Quantal noise from human red cone pigment. Nature Neuroscience. 11(5):565-71 (* equal contributions)
6) Xue T, Siu CW, Lieu DK, Lau CP, Tse HF, Li RA. (2007) Mechanistic role of I(f) revealed by induction of ventricular automaticity by somatic gene transfer of gating-engineered pacemaker (HCN) channels. Circulation. 115(14):1839-1850.
7) Tse HF, Xue T, Lau CP, Siu CW, Wang K, Zhang QY, Tomaselli GF, Akar FG, Li RA (2006). A bio-artificial sinus node constructed via in vivo gene transfer of an engineered pacemaker (HCN) channel reduces the dependence on electronic pacemaker in a sick sinus syndrome model. Circulation 114: 1000-1011. Issue highlights, GROUND-BREAKING STUDY OF 2007 AHA.
8) Xue T*, Cho H*, Akar F*, Tsang SY, Jones S, Marbán E, Tomaselli GF, Li RA (2005). Functional Integration of Electrically Active Cardiac Derivatives from Genetically Engineered Human Embryonic Stem Cells with Quiescent Recipient Ventricular Cardiomyocytes. Insights into the Development of Cell-based Pacemakers. Circulation. 111:11-20. issue highlights, (Best Paper Award, Circulation. 2005) (* equal contribution)
9) Wang G*, Xue T*, Tsang SY, Wong J, Cheng L, Zhang J, Li GR, Lau CP, Li RA, Tse HF (2005). Electrophysiological properties of pluripotent human and mouse embryonic stem cells. Stem Cells. 23(10):1526-34. (* equal contributions)
10) Azene EM*, Xue T*, Li RA (2003). Molecular basis of the effects of potassium on heterologously-expressed pacemaker (HCN) channels. J. Physiol. (London) 547(2):349 -356. (* equal contributions )
11) Xue T, Li RA (2002). An external determinant in the S5-P linker of the pacemaker (HCN) channel identified by sulfhydryl modification. J. Biol. Chem. 277(48):46233-42.
12) Xue T, Marbán E, Li RA (2002). Dominant-negative suppression of HCN1- and HCN2-encoded pacemaker currents by an engineered HCN1 construct: Insights into structure-function relationships and multimerization. Circ. Res. 90:1267-1273.